title: Osteogenic potential of adipose mesenchymal stem cells is not correlated with aortic valve calcification
metadata.name: dc.contributor.author
metadata.value: Malashicheva, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Irtyuga, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kostina, Aleksandra
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Ignatieva, Elena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhiduleva, Ekaterina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Semenova, Daria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Golovkin, Alexey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gordeev, Mikhail
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Moiseeva, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kostareva, Anna
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2018-09-03T13:01:18Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2018-09-03T13:01:18Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2018-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Malashicheva, A., Irtyuga, O., Kostina, A., Ignatieva, E., Zhiduleva, E., Semenova, D., Golovkin, A., Gordeev, M., Moiseeva, O., and Kostareva, A. 2018. Osteogenic potential of adipose mesenchymal stem cells is not correlated with aortic valve calcification. Bio. Comm. 63(2): 117–122.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/spbu03.2018.204
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/14759
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Osteogenic transformation as a result of cellular plasticity could be both beneficial,
  in the case of bone formation, and hazardous, in the case of vascular calcification.
  Mechanisms driving vascular calcification remain poorly understood,
  while calcification of the vessels is one of the leading causes of morbidity and
  mortality. In particular, calcification of the aortic valve is a serious complication
  requiring surgical intervention. The mechanisms behind aortic valve calcification
  and the origin of cells driving osteogenic transformation of the aortic
  valve remain questionable. A circulating stem cell theory supports the view that
  pathologic calcification could originate not only from valve cells, but also from
  other sources. The aim of this study was to estimate the osteogenic potential of
  adipose tissue-derived mesenchymal stem cells (MSCs) from people with calcification
  of the aortic valve versus MSCs from healthy people; further, to compare
  the capacity of osteogenic differentiation between MSCs and valve interstitial
  cells (VICs) from healthy donors and patients with severe calcification of the
  aortic valve. MSCs and VICs were isolated from either healthy donors or from
  patients with aortic valve calcification. The cells were immunophenotyped for
  conventional MSC markers by flow cytometry. Osteogenic differentiation was
  induced by addition of specific osteogenic inductors to the culture medium.
  Osteogenic differentiation was assessed by alizarin red staining and by estimation
  of RUNX2 expression by qPCR. The MSCs of healthy donors were capable of
  efficient osteogenic differentiation, while MSCs of the patients with aortic valve
  calcification were not capable of osteogenic differentiation. We conclude that
  there is no correlation between the capacity of adipose MSCs to osteogenically
  transform and calcification of the aortic valve. Most likely, peripheral MSCs of
  adipose origin could not be a source of aortic valve calcification.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by
  the Russian Science Foundation (grant
  No. 18‑14-00152).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 63; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: stem cells
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: calcification of aortic valve
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: osteogenic differentiation
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Osteogenic potential of adipose mesenchymal stem cells is not correlated with aortic valve calcification
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 04-Malashicheva et al.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/14759/1/04-Malashicheva%20et%20al.pdf
resources.format: Adobe PDF
resources.size: 515,87 kB