title: EDTA influence on the DNA complexation with Cu2+
metadata.name: dc.contributor.author
metadata.value: Paston, Sofia V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Nikolaev, Anton I.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2017-03-20T15:26:26Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2017-03-20T15:26:26Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2016-12
metadata.lang: -
metadata.name: dc.identifier.other
metadata.value: 10.21638/11701/spbu04.2016.405
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/6106
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Interaction of copper ions with DNA in solution in the presence of EDTA is studied with
  the method of circular dichroism. The results show that the complex of DNA nucleobases
  with copper ions is destroyed at the EDTA addition to the solution. Investigation of the
  solutions of thymidine (Thy), CuCl2 and EDTA by the method of electrospray ionization
  mass spectrometry reveals the existence of compound complexes [Y4−] · Cu2+ · Na+ · 2H+ · Thy,
  2{[Y4−] · Cu2+ · Na+ · 2H+ · Thy} and 2{[Y4−] · Cu2+ · 3Na+ · Thy}, where [Y4−] is completely
  deprotonated form of EDTA. In all complexes observed the Thy : [Y4−] : Cu2+ binding stoichiometry
  is 1 : 1 : 1. One can expect the appearance of nucleobases complexes with Cu2+ and EDTA
  in DNA sites with destabilised secondary structure as a result of heat motion of intact DNA, or in
  ligand binding locus, and also in the sites with primary structure defects. In the DNA solutions
  with Cu2+ or with Cu2+ and EDTA the increase in DNA secondary structure disturbance under
  the action of UVC light is observed. Refs 44. Figs 5. Tables 1.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: Работа выполнена при поддержке РФФИ (грант № 15-08-06876).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: ru
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Vestnik of St Petersburg University. Series 4. Physics. Chemistry;Vol. 3 (61); Issue 4
metadata.lang: -
metadata.name: dc.subject
metadata.value: DNA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: EDTA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: copper ions
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: circular dichroism
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: electrospray ionization mass spectrometry
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: EDTA influence on the DNA complexation with Cu2+
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 05-Paston.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/6106/1/05-Paston.pdf
resources.format: Adobe PDF
resources.size: 518,46 kB