title: Isolation of high-quality RNA from plant seeds
metadata.name: dc.contributor.author
metadata.value: Mishko, Alisa
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sundyreva, Maria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Stepanov, Ilya
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Efimenko, Sergey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Plotnikov, Vladimir
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Nenko, Natalia
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-08-20T19:59:56Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-08-20T19:59:56Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Mishko, A., Sundyreva, M., Stepanov, I., Efimenko, S., Plotnikov, V., and Nenko, N. 2021. Isolation of high-quality RNA from plant seeds. Bio. Comm. 66(2): 144–150.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.205
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/32816
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The apple (Malus domestica Borkh.) is one of the major fruit tree crops, but it
  hasn’t been well-studied as a breeding object for molecular investigations. It is
  important to develop reliable and rapid methods that allow the preparation of
  plant material for future research. We introduce a quick and simple method for
  isolating high-quality RNA from lipid-rich apple seeds (M. domestica cv. Golden
  Delicious). Our method does not employ highly toxic reagents, because we exclude
  phenol, 2-mercaptoethanol and others. The chemical composition of the
  extraction buffer is simple and has a minimum level of toxicity. We showed
  that, in chaotropic conditions (i.e., with lithium chloride-urea), silica (SiO2) can
  bind with the lipids and RNA will remain in the solution. The extracted RNA was
  of high quality and we successfully used it for synthesizing cDNA and RT-PCR.
  The protocol developed by us can be useful for researchers working with RNA
  extraction from plant seeds.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: Malus domestica
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: seeds
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: RNA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: lipids
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: silica
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: RT-PCR
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Isolation of high-quality RNA from plant seeds
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 144-150.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/32816/1/144-150.pdf
resources.format: Adobe PDF
resources.size: 866,06 kB