title: Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia
metadata.name: dc.contributor.author
metadata.value: Khadem, Azadeh
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bagheri, Abdolreza
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Moshtaghi, Nasrin
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Keykha Akhar, Fatemeh
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sharifi, Ahmad
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2022-10-25T17:30:24Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2022-10-25T17:30:24Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2022-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Khadem, A., Bagheri, A., Moshtaghi, N., Keykha Akhar, F., and Sharifi, A. 2022. Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia. Bio. Comm. 67(3): 160–167. https://doi.org/10.21638/spbu03.2022.303
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2022.303
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/38348
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Hairy root culture is a promising approach to improve production of plant secondary
  metabolites. The genes, which are located in T-DNA of a root-inducing
  plasmid, regulate auxin sensitivity of hairy roots. Therefore, this study was
  aimed to improve the growth and rosmarinic acid production of Lavandula angustifolia
  hairy roots. Lateral branches of hairy roots were transferred to ½ MS
  and ½ B5 liquid media. To assess auxin sensitivity, indole-3-acetic acid (IAA)
  and indole-3-butyric acid (IBA) with four different concentrations (0, 0.1, 0.5 and
  1 mg/l) were also applied. The growth of hairy roots in ½ MS medium was twofold
  higher than in ½ B5 medium. In addition, both auxins were found to significantly
  improve the growth of hairy roots whereas non-transformed roots
  stopped growing in the presence of the auxins. The highest dry weight and
  rosmarinic acid production of hairy roots were obtained from ½ MS medium
  supplemented with IBA irrespective of its concentration. As a result, the hairy
  roots grown in ½ MS medium supplemented with IBA produced the maximum
  amount of rosmarinic acid (7.98 mg/g dry weight of hairy roots). This first report
  of rosmarinic acid production in L. angustifolia hairy roots provides new insights
  into the auxin sensitivity of L. angustifolia hairy roots.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 67; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: auxin
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: basal media
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: hairy roots
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: rosmarinic acid
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: TL-DNA
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 03.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/38348/1/03.pdf
resources.format: Adobe PDF
resources.size: 2,06 MB