title: Establishment of primary cell lines from tissues of the red-eared slider
metadata.name: dc.contributor.author
metadata.value: Kulak, Maria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Galkina, Svetlana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Saifitdinova, Alsu
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gaginskaya, Elena
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2020-01-23T11:05:22Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2020-01-23T11:05:22Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-12
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Kulak, M., Galkina, S., Saifitdinova, A., and Gaginskaya, E. 2019. Establishment of primary cell lines from tissues of the red-eared slider Bio. Comm. 64(4): 229–234.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2019.401
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/16945
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Primary cell lines of the red-eared slider were established from somatic slider
  tissues cultivated in appropriate medium for a certain period. Exponential cell
  growth started after 2 weeks of continuous cultivating. The cells were of various
  morphology resembling keratinocytes, fibroblasts, and melanocytes, some
  of them gathered to form dense conglomerates similar to so-called “embryoid
  bodies”. The cell lines were maintained for 3–7 passages before being taken for
  cytogenetic manipulations. Red-eared slider cell lines derived in this way can be
  cryopreserved and successfully recovered to provide a source of fixed cells for
  3D imaging as well as a source of chromosomes and chromatin fiber preparations
  for cytological and cytogenetic studies. The protocol for red-eared slider
  cells was developed on the basis of the experiments described.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The research was sponsored
  by the Russian Foundation for Basic
  Research (project #18-04-01276A) and
  Saint Petersburg State University’s
  Programme 4 (project #1.40.1625.2017).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 64; Issue 4
metadata.lang: -
metadata.name: dc.subject
metadata.value: Trachemys scripta elegans
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: turtles
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: primary culture
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: explant
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Establishment of primary cell lines from tissues of the red-eared slider
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 229-234.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/16945/1/229-234.pdf
resources.format: Adobe PDF
resources.size: 528,74 kB