title: Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants
metadata.name: dc.contributor.author
metadata.value: Leonova, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shumilina, Julia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kim, Ahyoung
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Frolova, Nadezhda
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Wessjohann, Ludger
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bilova, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Frolov, Andrej
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2022-10-25T17:56:42Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2022-10-25T17:56:42Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2022-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Leonova, T., Shumilina, J., Kim, A., Frolova, N., Wessjohann, L., Bilova, T., and Frolov, A. 2022. Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants. Bio. Comm. 67(3): 236–244. https://doi.org/10.21638/spbu03.2022.309
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2022.309
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/38354
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Due to the oncoming climate changes water deficit represents one of the
  most important abiotic stressors which dramatically affects crop productivity
  worldwide. Because of their importance as the principal source of food protein,
  legumes attract a special interest of plant scientists. Moreover, legumes
  are involved in symbiotic association with rhizobial bacteria, which is morphologically
  localized to root nodules. These structures are critical for fixation of
  atmospheric nitrogen and highly sensitive to drought. Therefore, new droughttolerant
  legume cultivars need to be developed to meet the growing food demand.
  However, this requires a comprehensive knowledge of the molecular
  mechanisms behind the plant stress response. To access these mechanisms,
  adequate and reliable drought stress models need to be established. The agarbased
  polyethylene glycol (PEG) infusion model allows a physiologically relevant
  reduction of soil water potential (Ψw), although it is restricted to seedlings and
  does not give access to proteomics and metabolomics studies. Earlier, we successfully
  overcame this limitation and optimized this model for mature Arabidopsis
  plants. Here we make the next step forward and address its application
  to one of the major crop legumes — pea. Using a broad panel of physiological
  and biochemical markers, we comprehensively prove the applicability of this
  setup to legumes. The patterns of drought-related physiological changes are
  well-interpretable and generally resemble the stress response of plants grown
  in soil-based stop-watering models. Thus, the proposed model can be efficiently
  used in the study of stress-related metabolic adjustment in green parts, roots
  and root nodules of juvenile and flowering plants.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was supported by the
  Russian Foundation of Basic Research
  (projects No. 20-34-90160 and No. 19-316-
  51014).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 67; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: agar-based PEG infusion model
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: biochemical stress markers
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: drought
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: osmotic stress
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: oxidative stress
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: physiological stress markers
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: polyethylene glycol (PEG)
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 09.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/38354/1/09.pdf
resources.format: Adobe PDF
resources.size: 1,49 MB