title: Homoarginine and ornithine production during C2C12 myogenic differentiation
metadata.name: dc.contributor.author
metadata.value: Zhloba, Alexandr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Subbotina, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Prikhodko, Stanislava
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Khudiakov, Alexandr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Smolina, Natalia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kostareva, Anna
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2018-12-05T13:52:23Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2018-12-05T13:52:23Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2018-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Zhloba, A., Subbotina, T., Prikhodko, S., Khudiakov, A., Smolina, N., and Kostareva, A. 2018. Homoarginine and ornithine production during C2C12 myogenic differentiation. Bio. Comm. 63(3): 174–179.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/spbu03.2018.303
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/15101
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Assessment of cellular rates of amino acid consumption and release in vitro
  allows the study of cell culture in a time-course experiment without any cell
  damage. Determination of the release of amino acid metabolites that initially
  were not present in the media provides more reliable information about the
  processes of growth and differentiation in comparison with determination of
  amino acid consumption rates. Homoarginine (hArg), a derivative of arginine, is
  generated as the minor product in the reaction catalyzed by L-Arginine: glycine
  amidinotransferase, where L-lysine serves as an acceptor for amidine group
  instead of glycine. Ornithine is another product generated in this reaction from
  arginine. Thus, the goal of the present study was to evaluate the rate of hArg
  and ornithine accumulation in comparison to the rate of consumption of other
  amino acids in the course of C2C12 myoblast differentiation. The release time
  profiles were similar for hArg and ornithine, with the maximum corresponding
  to the second day of differentiation. The shift for hArg at this time point was
  detected with greater reliability (p < 0.002) than for ornithine and other amino
  acids. We suggest that hArg and ornithine could serve as markers to monitor
  the processes of myoblasts growth and differentiation.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by the
  Russian Science Foundation (14-15-00745П).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 63; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: amino acids
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: homoarginine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: myogenic differentiation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: ornithine
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Homoarginine and ornithine production during C2C12 myogenic differentiation
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 03-Zhloba.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/15101/1/03-Zhloba.pdf
resources.format: Adobe PDF
resources.size: 1,45 MB