title: Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic
metadata.name: dc.contributor.author
metadata.value: Karlov, Denis
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sazanova, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kuznetsova, Irina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tikhomirova, Nina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Popova, Zhanna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Osledkin, Yuriy
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Demidov, Nikita
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Belimov, Andrey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Safronova, Vera
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-04-16T18:33:35Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-04-16T18:33:35Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Karlov, D., Sazanova, A., Kuznetsova, I., Tikhomirova, N., Popova, Zh., Osledkin, Yu., Demidov, N., Belimov, A., and Safronova, V. 2021. Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic. Bio. Comm. 66(1): 73–82.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.109
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/27937
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Twenty-nine strains were isolated from two samples of the permafrost active
  layer of the Spitsbergen archipelago. The estimated number of bacteria ranged
  from 4.0⋅104 to 1.7⋅107 CFU∙g–1. As a result of sequencing of the 16S rRNA (rrs)
  genes, the isolates were assigned to 13 genera belonging to the phyla Actinobacteria,
  Proteobacteria (classes α, β, and γ), Bacteroidetes, and Firmicutes.
  Six isolates belonged to the rhizobial genus Mesorhizobium (order Rhizobiales).
  A plant nodulation assay with seedlings of legume plants Astragalus norvegicus,
  A. frigidus, A. subpolaris and Oxytropis sordida, originated from Khibiny (Murmansk
  region, Russia) and inoculated with Mesorhizobium isolates, showed the
  inability of these strains to form nodules on plant roots. Symbiotic (sym) genes
  nodC and nodD were not detected in Mesorhizobium strains either.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was performed with
  Russian Science Foundation support (grant
  No. 20-76-10042). Sampling, chemical
  analysis of samples and isolation of strains
  were supported by the Russian Science
  Foundation (grant No. 19-77-1006). The
  long-term storage of strains was supported
  by the Program for the Development
  and Inventory of Bioresource Collections.
  The research was performed using
  equipment of the Core Centrum “Genomic
  Technologies, Proteomics and Cell Biology”
  in ARRIAM.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 1
metadata.lang: -
metadata.name: dc.subject
metadata.value: legume plants
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: root nodule bacteria
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: 16S rRNA genes
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: permafrost soil
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Spitsbergen
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Rhizobial isolates in active layer samples of permafrost soil of Spitsbergen, Arctic
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 73-82.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/27937/1/73-82.pdf
resources.format: Adobe PDF
resources.size: 1,05 MB