title: Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes
metadata.name: dc.contributor.author
metadata.value: Maksiutenko, Evgeniia M
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Barbitoff, Yury A
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Matveenko, Andrew G
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Moskalenko, Svetlana E
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhouravleva, Galina A
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2022-06-14T20:12:29Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2022-06-14T20:12:29Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-12-12
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.3390/genes12122019
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.3390/genes12122019
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/36678
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Protein synthesis (translation) is one of the fundamental processes occurring in the cells of living organisms. Translation can be divided into three key steps: initiation, elongation, and termination. In the yeast Saccharomyces cerevisiae, there are two translation termination factors, eRF1 and eRF3. These factors are encoded by the SUP45 and SUP35 genes, which are essential; deletion of any of them leads to the death of yeast cells. However, viable strains with nonsense mutations in both the SUP35 and SUP45 genes were previously obtained in several groups. The survival of such mutants clearly involves feedback control of premature stop codon readthrough; however, the exact molecular basis of such feedback control remain unclear. To investigate the genetic factors supporting the viability of these SUP35 and SUP45 nonsense mutants, we performed whole-genome sequencing of strains carrying mutant sup35-n and sup45-n alleles; while no common SNPs or indels were found in these genomes, we discovered a systematic increase in the copy number of the plasmids carrying mutant sup35-n and sup45-n alleles. We used the qPCR method which confirmed the differences in the relative number of SUP35 and SUP45 gene copies between strains carrying wild-type or mutant alleles of SUP35 and SUP45 genes. Moreover, we compare the number of copies of the SUP35 and SUP45 genes in strains carrying different nonsense mutant variants of these genes as a single chromosomal copy. qPCR results indicate that the number of mutant gene copies is increased compared to the wild-type control. In case of several sup45-n alleles, this was due to a disomy of the entire chromosome II, while for the sup35-218 mutation we observed a local duplication of a segment of chromosome IV containing the SUP35 gene. Taken together, our results indicate that gene amplification is a common mechanism of adaptation to nonsense mutations in release factor genes in yeast.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: RSF grant 18-14-00050; State Research Program (0112-2016-0015)
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: translation termination
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: plasmid copy number
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: chromosome instability
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: WGS
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 2021 Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/36678/1/2021%20Gene%20Amplification%20as%20a%20Mechanism%20of%20Yeast%20Adaptation%20to%20Nonsense%20Mutations%20in%20Release%20Factor%20Genes.pdf
resources.format: Adobe PDF
resources.size: 612,26 kB