title: Stepwise injection potentiometric determination of caffeine in saliva using single-drop microextraction combined with solvent exchange
metadata.name: dc.contributor.author
metadata.value: Timofeeva, Irina I
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Medinskaia, Kseniya
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Nikolaeva, Larisa
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kirsanov, Dmirty
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bulatov, Andrey V.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2016-04-13T09:53:51Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2016-04-13T09:53:51Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2016-04-01
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.1016/j.talanta.2016.01.001
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.1016/j.talanta.2016.01.001
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.1016/j.talanta.2016.01.001
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/2085
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: A flow potentiometric method for determination of caffeine in saliva is suggested. This task is important for non-invasive assessment of drug metabolizing system activity in hepatocytes. In the current study, stepwise injection analysis (SWIA) was successfully combined with single-drop liquid microextraction (SDLME) and solvent exchange procedure. The method is based on the caffeine SDLME with subsequent solvent evaporation and dissolution of analyte in sulfuric acid followed by potentiometric detection using poly(vinyl chloride) membrane electrode containing potassium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate as electroactive component. SDLME was employed for elimination of interfering matrix effects of saliva and caffeine metabolites such as theophylline, theobromine and paraxanthine. A linear range of 10(-5)-10(-2)M was established for caffeine with detection limit at 6×10(-6)M. The sample throughput was 6 samples h(-1). The proposed method was successfully applied to the determination of caffeine in saliva and the analytical results agreed well with the results obtained with reference HPLC method.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: Russian Foundation Basic Research (projects No14-03-31092 mol_a, 15-33-20068 and 3116-33-60126/15).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Elsevier
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Talanta;Vol. 150
metadata.lang: -
metadata.name: dc.subject
metadata.value: Caffeine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Saliva
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Single-drop liquidmicroextraction
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Stepwiseinjectionanalysis
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Caffeine sensor
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Stepwise injection potentiometric determination of caffeine in saliva using single-drop microextraction combined with solvent exchange
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Talanta 2016_V150.docx
resources.url: https://dspace.spbu.ru/bitstream/11701/2085/1/Talanta%202016_V150.docx
resources.format: Microsoft Word XML
resources.size: 14,82 kB