title: GC-MS determination of hydroxylamine in blood and urine
metadata.name: dc.contributor.author
metadata.value: Ukolov, A. I.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Radilov, A. S.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2017-10-28T15:28:57Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2017-10-28T15:28:57Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2017-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Ukolov A. I., RadilovA. S. GC-MS determination of hydroxylamine in blood and urine. Vestnik SPbSU. Physics and Chemistry. 2017. Vol. 4 (62), iss. 3. P. 337–345.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/11701/spbu04.2017.308
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/8633
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: A GC-MS method was developed for hydroxylamine (HA) quantitation in blood plasma
  and urine. Feature of this method is two stages of derivatization: benzaldehyde and bistrimethylsilyltrifluoroacetamide.
  HA is a highly polar, hydrophilic, low-weight basic compound.
  We tried a variety of approaches to derivatization and isolation of HA from water phase: simultaneous
  extraction and derivatization HA with acetone, extraction of acetone oxime using
  head-space solid phase microextraction, benzaldehyde derivatization and extraction into an organic
  phase of the resulting oxime and finally, a twin stage derivatization with benzaldehyde and
  bis-trimethylsilyltrifluoroacetamide, which has been recognized by us the best option. LOD of
  GC-MS method is 30 ng/mL, but using GC-MS/MS allow us to reduce LOD to 0.1 ng/mL. Quantification
  was performed by using relative calibration with methoxyamine as internal standard.
  Toxicological experiments with experimental animals were carried out in order to establishing
  possibilities of our method for diagnostics of acute and chronic intoxication of hydroxylamine. It was shown that GC-MS are possible to diagnose just acute poisoning hydroxylamine (SLD50),
  but GC-MS/MS are possible to diagnose chronic intoxication by HA (2 mg/L in drinking water).
  Refs 16. Figs 4. Tables 2.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: ru
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Vestnik of St Petersburg University. Physics and Chemistry;Vol. 4 (62); Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: hydroxylamine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: blood
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: urine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: jet fuel
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: GC-MS
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: GC-MS determination of hydroxylamine in blood and urine
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 08-Ukolov.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/8633/1/08-Ukolov.pdf
resources.format: Adobe PDF
resources.size: 350,72 kB