title: New Insights Into Chromomere Organization Provided by Lampbrush Chromosome Microdissection and High-Throughput Sequencing
metadata.name: dc.contributor.author
metadata.value: Zlotina, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Maslova, Antonina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Pavlova, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kosyakova, Nadezda
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Al-Rikabi, Ahmed
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Liehr, Thomas
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Krasikova, Alla
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2020-10-26T14:33:10Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2020-10-26T14:33:10Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2020-02
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.3389/fgene.2020.00057
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Front Genet. 2020; 11:57.
metadata.lang: en_GB
metadata.name: dc.identifier.issn
metadata.value: 10.3389/fgene.2020.00057
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.3389/fgene.2020.00057
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/20092
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Giant lampbrush chromosomes (LBCs) typical for growing oocytes of various animal species are characterized by a specific chromomere-loop appearance and massive transcription. Chromomeres represent universal units of chromatin packaging at LBC stage. While quite good progress has been made in investigation of LBCs structure and function, chromomere organization still remains poorly understood. To extend our knowledge on chromomere organization, we applied microdissection to chicken LBCs. In particular, 31 and 5 individual chromomeres were dissected one by one along the macrochromosome 4 and one microchromosome, respectively. The data on genomic context of individual chromomeres was obtained by high-throughput sequencing of the corresponding chromomere DNA. Alignment of adjacent chromomeres to chicken genome assembly provided information on chromomeres size and genomic boarders, indicating that prominent marker chromomeres are about 4-5 Mb in size, while common chromomeres of 1.5-3.5 Mb. Analysis of genomic features showed that the majority of chromomere-loop complexes combine gene-dense and gene-poor regions, while massive loopless DAPI-positive chromomeres lack genes and are remarkably enriched with different repetitive elements. Finally, dissected LBC chromomeres were compared with chromatin domains (topologically associated domains [TADs] and A/B-compartments), earlier identified by Hi-C technique in interphase nucleus of chicken embryonic fibroblasts. Generally, the results obtained suggest that chromomeres of LBCs do not correspond unambiguously to any type of well-established spatial domains of interphase nucleus in chicken somatic cells.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Frontiers in Genetics
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: New Insights Into Chromomere Organization Provided by Lampbrush Chromosome Microdissection and High-Throughput Sequencing
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Zlotina_et_al.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/20092/1/Zlotina_et_al.pdf
resources.format: Adobe PDF
resources.size: 2,05 MB