title: Nonsense Mutations in the Yeast SUP35 Gene Affect the [PSI+] Prion Propagation
metadata.name: dc.contributor.author
metadata.value: Trubitsina, Nina P
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zemlyanko, Olga M
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bondarev, Stanislav A
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhouravleva, Galina A
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2020-03-27T12:56:24Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2020-03-27T12:56:24Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2020-02-28
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.3390/ijms21051648
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.3390/ijms21051648
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/17290
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The essential SUP35 gene encodes yeast translation termination factor eRF3. Previously, we isolated nonsense mutations sup35-n and proposed that the viability of such mutants can be explained by readthrough of the premature stop codon. Such mutations, as well as the prion [PSI+], can appear in natural yeast populations, and their combinations may have different effects on the cells. Here, we analyze the effects of the compatibility of sup35-n mutations with the [PSI+] prion in haploid and diploid cells. We demonstrated that sup35-n mutations are incompatible with the [PSI+] prion, leading to lethality of sup35-n [PSI+] haploid cells. In diploid cells the compatibility of [PSI+] with sup35-n depends on how the corresponding diploid was obtained. Nonsense mutations sup35-21, sup35-74, and sup35-218 are compatible with the [PSI+] prion in diploid strains, but affect [PSI+] properties and lead to the formation of new prion variant. The only mutation that could replace the SUP35 wild-type allele in both haploid and diploid [PSI+] strains, sup35-240, led to the prion loss. Possibly, short Sup351-55 protein, produced from the sup35-240 allele, is included in Sup35 aggregates and destabilize them. Alternatively, single molecules of Sup351-55 can stick to aggregate ends, and thus interrupt the fibril growth. Thus, we can conclude that sup35-240 mutation prevents [PSI+] propagation and can be considered as a new pnm mutation.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by grants from Russian Science Foundation [grant 18-14-00050] (plasmid construction, fluorescence microscopy, mutation analysis in haploids) and Russian Foundation for Basic Research [grant 17-54-150002] (individual mutation analysis in diploids). Technical help was provided by Resource Center “Development of Molecular and Cell Technologies” of St. Petersburg State University. Authors declare no conflict of interest.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: MDPI
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: yeast
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: S.cerevisiae
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: prion
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: [PSI+]
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: translation termination
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: nonsense mutations
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Sup35
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Nonsense Mutations in the Yeast SUP35 Gene Affect the [PSI+] Prion Propagation
metadata.lang: en_GB
metadata.name: dc.title.alternative
metadata.value: Нонсенс-мутации в дрожжевом гене SUP35 влияют на распространение приона [PSI+]
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: ijms-21-01648-v2.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/17290/1/ijms-21-01648-v2.pdf
resources.format: Adobe PDF
resources.description: Article
resources.size: 7,13 MB
resources.filename: ijms-21-01648-s001.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/17290/2/ijms-21-01648-s001.pdf
resources.format: Adobe PDF
resources.description: Supporting information
resources.size: 803,42 kB