title: Rye cytogenetics and chromosome genomics
metadata.name: dc.contributor.author
metadata.value: Mikhailova, Elena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Doležel, Jaroslav
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2023-02-13T11:51:00Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2023-02-13T11:51:00Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Mikhailova E., Doležel J. Rye cytogenetics and chromosome genomics. In M.T.Rabanus-Wallace and N.Stein (Eds.) The Rye Genome, Compendium of Plant Genomes. Springer Nature Switzerland. 2021. p.43-62. DOI: DOI:10.1007/978-3-030-83383-1_3
metadata.lang: en_GB
metadata.name: dc.identifier.issn
metadata.value: 2199-479X (electronic)
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 2199-4781
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/39091
metadata.lang: -
metadata.name: dc.description
metadata.value: The book series "Compendium of Plant Genomes" ISBN 978-3-030-83382-4; ISBN 978-3-030-83383-1 (eBook)
metadata.lang: en_GB
metadata.name: dc.description.abstract
metadata.value: Rye (Secale cereale L., 2n = 2x = 14) has a large genome of about 8 Gbp distributed across seven large chromosomes. Although they are easily observable by microscopy, their identification is difficult due to similar morphology.
  Thus, wheat-rye chromosome addition and substitution lines were originally employed to accomplish this and establish the homology of rye chromosomes with those of Triticinae species. The introduction of differential staining and fluorescence in
  situ hybridization (FISH) provided an important advance, but chromosome identification was still hampered by polymorphism of chromosome banding patterns. A different approach to identify chromosomes involves crosses of a sample to a tester set of wheat-rye chromosome addition lines and cytological analysis of chromosome pairing during meiosis in F1 hybrids. While FISH enabled the analysis of long-range molecular organization of the chromosomes, genomic in situ hybridization (GISH) using rye genomic DNA as probe allowed identification of rye chromosomes introgressed to wheat, including interspecific chromosome translocations. The analysis of isolated mitotic metaphase chromosomes by flow
  cytometry enabled identification of chromosome 1R, and, if present, the accessory B chromosome. The two chromosomes could be purified by flow sorting for downstream analyses. Chromosomes 2R–7R could not be discriminated from each other and thus were flow-sorted individually from respective wheat-rye chromosomes addition lines, as was the short
  arm of 1R (1RS).Flow sorting of rye chromosomes facilitated the development of chromosome-specific molecular markers. Next generation sequencing of flow-sorted B chromosomes provided insights into their molecular organization and origin (Chap. 4). Sequencing each of the seven rye chromosomes resulted in the first draft genome sequence, informing about rye gene complement and evolution, and recently facilitated the assembly of a rye
  reference genome.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The authors are grateful to Ekaterina D. Badaeva for providing figures with C-banded and FISH probed mitotic chromosomes of different rye species. JD was supported by ERDF project “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827)
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Springer Nature Switzerland AG
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Rye genome;Chapter 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: Chromosome morphology, Chromosome sequencing, Differential staining, FISH
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Flow-cytometric sorting, GISH, Homoeologous chromosome groups, Molecular marker development, Physical mapping, Wheat-rye chromosome introgressions
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Rye cytogenetics and chromosome genomics
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Book chapter
metadata.lang: en_GB
resources.filename: 978-3-030-83383-1_3.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/39091/2/978-3-030-83383-1_3.pdf
resources.format: Adobe PDF
resources.description: The Rye Genome Ch3
resources.size: 854,72 kB