title: Bioavailability Study of Recombinant Plasmid DNA Nerve Growth Factor after Intramuscular Injection by Electroporation
metadata.name: dc.contributor.author
metadata.value: Shilov, Viktor V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Yudin, Mikhail A.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Vengerovich, Nikolai G.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shcherbakov, Taras V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bogacheva, Aleksandra S.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2019-11-26T12:36:06Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2019-11-26T12:36:06Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Shilov V. V., Yudin M. A., Vengerovich N. G., Shcherbakov T. V., Bogacheva A. S. Bioavailability Study of Recombinant Plasmid DNA Nerve Growth Factor after Intramuscular Injection by Electroporation. Vestnik of Saint Petersburg University. Medicine, 2019, vol. 14, issue 1, pp. 89– 97.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu11.2019.201
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/16647
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Comparative analysis of plasmid DNA delivery into cells efficiency (with effective agent concentration
  250 μg/kg), ensuring accumulation of nerve growth factor (NGF) after intramuscular
  injection and intramuscular administration using electroporation method on rats was performed.
  Application of electroporation method ensured over 4 times increase of DNA plasmid
  concentration in muscle tissue within the first hour after its injection and increased the
  duration of its determination. Higher bioavailability parameters were confirmed by 3 times
  increase in gene expression level, encoding NGF accumulation and 3 times increase in concentration
  of the target NGF protein in blood plasma. The results obtained allow us to recommend
  this method for introduction of NGF plDNA in its biological activity and effectiveness
  in models of nerve damage investigation.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Vestnik of St Petersburg University. Medicine;Volume 14; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: plasmid DNA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: pharmacokinetics
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: gene expression
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: bioavailability
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: electroporation
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Bioavailability Study of Recombinant Plasmid DNA Nerve Growth Factor after Intramuscular Injection by Electroporation
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 89-97.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/16647/1/89-97.pdf
resources.format: Adobe PDF
resources.size: 740,3 kB