title: Isolation, culturing and 3D bioprinting of equine myoblasts
metadata.name: dc.contributor.author
metadata.value: Aimaletdinov, Alexander
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Abyzova, Maria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kurilov, Igor
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Yuferova, Alina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Rutland, Catrin
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Rizvanov, Albert
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zakirova, Elena
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2022-10-25T17:24:16Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2022-10-25T17:24:16Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2022-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Aimaletdinov, A., Abyzova, M., Kurilov, I., Yuferova, A., Rutland, C., Rizvanov, A., and Zakirova, E. 2022. Isolation, culturing and 3D bioprinting of equine myoblasts. Bio. Comm. 67(3): 152–159. https://doi.org/10.21638/spbu03.2022.302
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2022.302
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/38347
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Isolating and culturing myoblasts is essential for techniques such as tissue regeneration
  and in vitro meat production. This research describes a protocol to
  isolate primary myoblasts from skeletal muscle of an adult horse. The equine
  primary myoblasts expressed markers specific to myoblasts and had multipotent
  potential capabilities with differentiation into chondrocytes, adipocytes
  and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and
  grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies
  showed that the use of GelMa bioink and ionic cross-linking did not have
  negative effects on cell proliferation at the beginning of cultivation. However,
  cells showed reduced proliferative activity by day 40 following in vitro culturing.
  The population of primary equine myoblasts obtained from an adult individual,
  and propagated on microcarriers and bioink, did not meet the requirements
  of the regenerative veterinary and manufacturing meat in vitro regarding the
  quantity and quality of the cells required. Nonetheless, further optimization of
  the cell scaling up process, including both microcarriers and/or the bioreactor
  program and bioprinting, is still important.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This paper was supported
  by the Kazan Federal University
  Strategic Academic Leadership Program
  (PRIORITY-2030).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 67; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: myoblasts
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: bioprinting
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: microcarriers
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Isolation, culturing and 3D bioprinting of equine myoblasts
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 02.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/38347/2/02.pdf
resources.format: Adobe PDF
resources.size: 2,86 MB