title: A fully automated effervescence-assisted switchable solvent-based liquid phase microextraction procedure: Liquid chromatographic determination of ofloxacin in human urine samples
metadata.name: dc.contributor.author
metadata.value: Vakh, Christina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Pochivalov, Aleksei
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Andruch, Vasil
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Moskvin, Leonid
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bulatov, Andrey
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2016-04-17T20:34:51Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2016-04-17T20:34:51Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2016-02-11
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.1016/j.aca.2015.12.004
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Analytica Chimica Acta 907 (2016) 54e59
metadata.lang: en_GB
metadata.name: dc.identifier.issn
metadata.value: 10.1016/j.aca.2015.12.004
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/2116
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: A novel fully automated effervescence-assisted switchable solvent-based liquid phase microextraction procedure has been suggested. In this extraction method, medium-chain saturated fatty acids were investigated as switchable hydrophilicity solvents. The conversion of fatty acid into hydrophilic form was carried out in the presence of sodium carbonate. The injection of sulfuric acid into the solution decreased the pH value of the solution, thus, microdroplets of the fatty acid were generated. Carbon dioxide bubbles were generated in-situ, and promoted the extraction process and final phase separation. The performance of the suggested approach was demonstrated by the determination of ofloxacin in human urine samples using high-performance liquid chromatography with fluorescence detection. This analytical task was used as a proof-of-concept example. Under the optimal conditions, the detector response of ofloxacin was linear in the concentration ranges of 3·10(-8)-3·10(-6) mol L(-1). The limit of detection, calculated from a blank test based on 3σ, was 1·10(-8) mol L(-1). The results demonstrated that the presented approach is highly cost-effective, simple, rapid and environmentally friendly.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by the Russian Foundation for Basic
  Research (projects no. 13e03e00031ea, no. 15-33-20068, no. 16-
  33-60137-mol_a_dk, 16-33-00037-mol_a), Scientific Grant Agency
  of the Ministry of Education of the Slovak Republic and the Slovak
  Academy of Sciences (Grant no. VEGA 1/0010/15). Scientific research was performed at the Center for Chemical Analysis and
  Materials Research and the Educational Resource Center of
  Research Park of St.Petersburg State University. This work was
  supported in part through the development of bilateral cooperation
  between the Slovak and Russian universities within the Operational
  Programme Education financed by the European Social Fund (ESF)
  (project ITMS 26110230084).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Elsevier
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Effervescence-assisted switchable solventbased liquid phase microextraction
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Green analytical chemistry
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Flow analysis
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Fatty acids
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Ofloxacin
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Urine
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: A fully automated effervescence-assisted switchable solvent-based liquid phase microextraction procedure: Liquid chromatographic determination of ofloxacin in human urine samples
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Ofloxacin_ACA_2016.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/2116/1/Ofloxacin_ACA_2016.pdf
resources.format: Adobe PDF
resources.size: 996,81 kB