title: Protein glycation and drought response of pea (Pisum sativum L.) root nodule proteome: a proteomics approach
metadata.name: dc.contributor.author
metadata.value: Shumilina, Julia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gorbach, Daria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Popova, Veronika
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tsarev, Alexander
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kusnetsova, Alena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Grashina, Maria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Dorn, Mandy
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Lukasheva, Elena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Osmolovskaya, Natalia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Romanovskaya, Ekaterina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhukov, Vladimir
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Ihling, Christian
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Grishina, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bilova, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Frolov, Andrej
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-12-10T12:17:03Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-12-10T12:17:03Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Shumilina, J., Gorbach, D., Popova, V., Tsarev, A., Kuznetsova, A., Grashina, M., Dorn, M., Lukasheva, E., Osmolovskaya, N., Romanovskaya, E., Zhukov, V., Ihling, C., Grishina, T., Bilova, T., and Frolov, A. 2021. Protein glycation and drought response of pea (Pisum sativum L.) root nodule proteome: a proteomics approach. Bio. Comm. 66(3): 210–224.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.303
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/33814
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Because of ongoing climate change, drought is becoming the major factor limiting
  productivity of all plants, including legumes. As these protein-rich crops form
  symbiotic associations with rhizobial bacteria — root nodules — they readily lose
  their productivity under drought conditions. Understanding the underlying molecular
  mechanisms might give access to new strategies to preserve the productivity
  of legume crops under dehydration. As was shown recently, development of
  drought response is accompanied by alterations in the patterns of protein glycation
  and formation of advanced glycation end products (AGEs) that might be a part
  of unknown regulatory mechanisms. Therefore, here we address the effects of
  moderate drought on protein dynamics and AGE patterns in pea (Pisum sativum)
  root nodules. For this, plants inoculated with rhizobial culture were subjected to
  osmotic stress for one week, harvested, the total protein fraction was isolated
  from root nodules by phenol extraction, analyzed by bottom-up LC-MS-based
  proteomics, and AGE patterns were characterized. Surprisingly, despite the clear
  drought-related changes in phenotype and stomatal conductivity, only minimal
  accompanying expressional changes (14 rhizobial and 14 pea proteins, mostly involved
  in central metabolism and nitrogen fixation) could be observed. However,
  71 pea and 97 rhizobial proteins (mostly transcription factors, ABC transporters
  and effector enzymes) were glycated, with carboxymethylation being the major
  modification type. Thereby, the numbers of glycated sites in nodule proteins dramatically
  decreased upon stress application. It might indicate an impact of glycation
  in regulation of transport, protein degradation, central, lipid and nitrogen
  metabolism. The data are available at Proteome Xchange (accession: PXD024042).
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This study was supported by
  the Russian Foundation of Basic Research
  (RFBR, 50 % of the work dealing with
  proteomics analysis and AGE profiling, grant
  No 18-016-190A) and the Russian Science
  Foundation (project No 20-16-00086, 50 %
  of the work dealing with the methodology,
  application and physiology of drought
  stress).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: advanced glycation end products (AGEs)
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: drought stress
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: glycation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: pea (Pisum sativum)
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: polyethylene glycol (PEG)
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: proteomics
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: root nodules
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Protein glycation and drought response of pea (Pisum sativum L.) root nodule proteome: a proteomics approach
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 10086-Article Text-38477-1-10-20211112.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/33814/1/10086-Article%20Text-38477-1-10-20211112.pdf
resources.format: Adobe PDF
resources.size: 3,57 MB