title: Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae
metadata.name: dc.contributor.author
metadata.value: Drozdova, Polina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Lipaeva, Polina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Rogoza, Tatyana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhouravleva, Galina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bondarev, Stanislav
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2018-03-10T19:59:50Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2018-03-10T19:59:50Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2018-03-01
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.1371/journal.pone.0193726
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.1371/journal.pone.0193726
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/9217
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The Sch9 kinase of Saccharomyces cerevisiae is one of the major TOR pathway effectors and regulates diverse processes in the cell. Sch9 belongs to the AGC kinase family. In human, amplification of AGC kinase genes is connected with cancer. However, not much is known about the effects of Sch9 overproduction in yeast cells. To fill this gap, we developed a model system to monitor subcellular location and aggregation state of overproduced Sch9 or its regions fused to a fluorescent protein. With this system, we showed that Sch9-YFP forms detergent-resistant aggregates, and multiple protein regions are responsible for this. This finding corroborated the fact that Sch9-YFP is visualized as various fluorescent foci. In addition, we found that Sch9 overproduction caused cell elongation, and this effect was determined by its C-terminal region containing kinase domains. The constructs we present can be exploited to create superior yeast-based model systems to study processes behind kinase overproduction in cancers.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 2018_Overproduction of Sch9 leads to its aggregation and cell elongation in Saccharomyces cerevisiae.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/9217/1/2018_Overproduction%20of%20Sch9%20leads%20to%20its%20aggregation%20and%20cell%20elongation%20in%20Saccharomyces%20cerevisiae.pdf
resources.format: Adobe PDF
resources.size: 1,61 MB