title: Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype
metadata.name: dc.contributor.author
metadata.value: Lutova, Ludmila
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Dodueva, Irina
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2019-08-28T21:49:41Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2019-08-28T21:49:41Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Lutova, L. and Dodueva, I. 2019. Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype. Bio. Comm. 64(2): 124–132.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2019.204
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/16208
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: This review highlights the years of research on the genetics of in vitro regeneration in higher plants conducted at the Department of Genetics and Biotechnology of Saint Petersburg State University. The genetic collection of radish (Raphanus sativus) created at the department by selfing of individual plants from three cultivars was used as a model in these studies. Some radish inbred lines from the genetic collection form spontaneous tumors in the roots and are also used to study mechanisms of tumor growth in higher plants. It was revealed that radish lines that differed in the ability to form tumors also contrastingly differed in the reaction of their explants to auxin and cytokinin in vitro, which reflects a difference in the levels of these hormones in the tissues of related tumorous and non-tumorous radish lines. Moreover, high concentrations of cytokinins in cultural medium induced tumor formation in the regenerated plants of tumorous radish lines. The presence of meristematic zones in spontaneous tumors in radish lines, as well as in crown gall tumors induced by Agrobacterium tumefaciens and cytokinin-induced tumors made it possible to reveal the role of the main meristem regulators, such as KNOX and WOX family transcription factors and the CLAVATA system, in both the process of tumor growth and regeneration in plants. Analysis of the expression of meristem-specific genes during the development of spontaneous and induced tumors in radish as well as in regenerated radish plants confirmed this assumption.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was supported by RSCF 16-16-10011 and the RFBR 17-04-01708 grants.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 64; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: Raphanus sativus
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: regeneration
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: tumors
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: meristems
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: phytohormones
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 124-132.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/16208/1/124-132.pdf
resources.format: Adobe PDF
resources.size: 669,76 kB