title: Enzyme activity of topsoil layer on reclaimed and unreclaimed post-mining sites
metadata.name: dc.contributor.author
metadata.value: Heděnec, Petr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Vindušková, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kukla, Jaroslav
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Šnajdr, Jaroslav
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Baldrian, Petr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Frouz, Jan
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2017-05-15T11:33:24Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2017-05-15T11:33:24Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2017-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Heděnec, P., Vindušková, O., Kukla, J., Šnajdr, J., Baldrian, P. & Frouz, J. 2017. Enzyme activity of topsoil layer on reclaimed and unreclaimed post-mining sites. Bio. Comm. 62(1): 19–25.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/11701/spbu03.2017.103
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/6287
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Topsoil layer contains various components of soil organic matter such as branches,
  leaves, bark, or metabolites and residues of soil biota. Soil organic matter (SOM)
  in forest ecosystems consists mostly of lignin, cellulose, chitin and other hydrocarbons.
  These compounds are decomposed mainly by soil fungi which produce
  extracellular enzymes to decompose wide range of organic residues. These enzymes
  may alter nutrient cycling and change soil properties such as water retention
  capacity, ion exchange capacity, formation of soil microstructure, soil microbial
  respiration, etc. In this study, we studied enzyme activity in the topsoil layer of postmining
  spoil heaps near Sokolov, Czech Republic. We investigated the effect of the
  following factors and their combinations on enzyme activity: i) dominant vegetation,
  ii) time of sampling, iii) reclamation, and iv) soil macrofauna.
  We measured enzyme activity in plastic mesocosms with autochthonous litter
  deposited on reclaimed and unreclaimed post-mining sites. We used mesocosms
  accessible for macrofauna (mesh size > 2 mm) and mesocosms inaccessible for
  macrofauna (mesh size < 2 mm). Under laboratory conditions, we measured enzyme
  activity (laccase, oxidase, peroxidase, Mn-peroxidase, alkaline phosphatase,
  acid phosphatase, endoglucanase, xylanase, cellobiohydrolase, glucosidase, xylosidase
  and chitinase) using buffer extraction method followed by spectrophotometric
  assay. We did not find any statistically significant difference between mesocosms
  accessible and inaccessible for fauna. However, we found significant effect of time
  of sampling on enzyme activity. Our results showed significant difference between
  reclaimed and unreclaimed post-mining sites. Reclaimed sites showed significantly
  higher enzyme activity than unreclaimed sites.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This study was supported by the Research
  Centre grant LC06066 and by grant from Czech
  Science Foundation P504/12/1288.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 62; Issue 1
metadata.lang: -
metadata.name: dc.subject
metadata.value: enzyme assay
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: microbial activity
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: litterbag
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: macrofauna
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: soil fauna
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Enzyme activity of topsoil layer on reclaimed and unreclaimed post-mining sites
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 03-Hedenec.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/6287/1/03-Hedenec.pdf
resources.format: Adobe PDF
resources.size: 2,41 MB