title: Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes
metadata.name: dc.contributor.author
metadata.value: Saifitdinova, Alsu
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Galkina, Svetlana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Volodkina, Valeriia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gaginskaya, Elena
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2018-02-28T14:40:18Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2018-02-28T14:40:18Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2017-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Saifitdinova, A., Galkina, S., Volodkina, V., and Gaginskaya E. 2017. Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes. Bio. Comm. 62(3): 165–168.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/11701/spbu03.2017.302
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/9202
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Lampbrush chromosomes represent transcriptionally active chromosomes that
  exist during the extended diplotene phase in the growing ovarian eggs of many
  vertebrate animals, except therian mammals. They are excellent for studying
  chromosome structure and aspects of RNA transcription. Because of their giant
  size they allow a great increase in the resolution for physical gene mapping.
  Lampbrush chromosomes have no connection with the nuclear envelope, and
  can be manually dissected from living oocyte nuclei. Here we present the complete
  protocol for lampbrush chromosome microsurgical isolation from avian
  and reptilian germinal vesicles. These chromosome preparations can be further
  used for fluorescence in situ hybridization (FISH) and immunostaining.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The research was carried out
  under project 15-04-05684 financed by
  Russian Basic Research Foundation.
  V. V. was supported by a grant from
  the Saint Petersburg State University
  (1.42.961.2016).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 62; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: nucleus
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: germinal vesicle
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: lampbrush chromosomes
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: meiotic chromosomes
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: microsurgical isolation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Aves
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Reptilia
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Preparation of lampbrush chromosomes dissected from avian and reptilian growing oocytes
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 02_Saifitdinova.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/9202/1/02_Saifitdinova.pdf
resources.format: Adobe PDF
resources.size: 4,75 MB