title: Human APP Gene Expression Alters Active Zone Distribution and Spontaneous Neurotransmitter Release at the Drosophila Larval Neuromuscular Junction
metadata.name: dc.contributor.author
metadata.value: Saburova, Ekaterina A.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Vasiliev, Alexander N.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kravtsova, Violetta V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Ryabova, Elena V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zefirov, Andrey L.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bolshakova, Olga I.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sarantseva, Svetlana V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Krivoi, Igor I.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2017-07-10T14:13:31Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2017-07-10T14:13:31Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2017-07-09
metadata.lang: -
metadata.name: dc.identifier
metadata.value: https://doi.org/10.1155/2017/9202584
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Neural Plasticity, Volume 2017, Article ID 9202584, 10 pages
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/6766
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: This study provides further insight into the molecular mechanisms that control neurotransmitter release. Experiments were performed on larval neuromuscular junctions of transgenic Drosophila melanogaster lines with different levels of human amyloid precursor protein (APP) production. To express human genes in motor neurons of Drosophila, the UAS-GAL4 system was used. Human APP gene expression increased the number of synaptic boutons per neuromuscular junction. The total number of active zones, detected by Bruchpilot protein puncta distribution, remained unchanged; however, the average number of active zones per bouton decreased. These disturbances were accompanied by a decrease in frequency of miniature excitatory junction potentials without alteration in random nature of spontaneous quantal release. Similar structural and functional changes were observed with co-overexpression of human APP and β-secretase genes. In Drosophila line with expression of human amyloid-β42 peptide itself, parameters analyzed did not differ from controls, suggesting the specificity of APP effects.These results confirm the involvement of APP in synaptogenesis and provide evidence to suggest that human APP overexpression specifically disturbs the structural and functional organization of active zone and results in altered Bruchpilot distribution and lowered probability of spontaneous neurotransmitter release.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.source
metadata.value: Neural Plasticity
metadata.lang: -
metadata.name: dc.title
metadata.value: Human APP Gene Expression Alters Active Zone Distribution and Spontaneous Neurotransmitter Release at the Drosophila Larval Neuromuscular Junction
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Saburova_9202584.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/6766/1/Saburova_9202584.pdf
resources.format: Adobe PDF
resources.size: 4,04 MB