title: Comparison of two systems of tonoplast purification from tobacco cells of suspension culture BY-2
metadata.name: dc.contributor.author
metadata.value: Chen, Тingzhuo
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kirpichnikova, Аnastasia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Mikhaylova, Yulia
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shishova, Maria
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2020-09-04T22:03:31Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2020-09-04T22:03:31Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2020-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Chen, Т., Kirpichnikova, A., Mikhaylova, Yu., Shishova, M. 2020. Comparison of two systems of tonoplast purification from tobacco cells of suspension culture BY-2. Bio. Comm. 65(2): 178–186.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/ spbu03.2020.204
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/19218
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The tonoplast is an intracellular membrane, important for numerous functions of the vacuoles in plant cells. Transport, signaling, enzymatic activity and other processes of the tonoplast are the focus of a wide spectrum of studies. Multiple advanced analyses demand highly purified vacuoles or vesicles of the tonoplast membrane fraction. Since 1960 several approaches have been developed for such purification, but new goals and new model objects require adjustment of already existing techniques. The presented investigation aimed to compare two methods of tonoplast membrane fraction purification from tobacco suspension cell culture BY-2 (Nicotiana tabacum L., cv Bright Yellow) based on sucrose and sucrose/sorbitol gradients. The functional activity of obtained purified tonoplast fractions was measured by the hydrolytic activity of vacuolar H+-ATPase and H+-PPase.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was partly supported by the Russian Foundation for Basic Research (No. 19-04-00655).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 65; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: tonoplast
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: vesicle fraction
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: purification
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: V-ATPase
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: H+-PPase
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Comparison of two systems of tonoplast purification from tobacco cells of suspension culture BY-2
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 178-186.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/19218/1/178-186.pdf
resources.format: Adobe PDF
resources.size: 1,8 MB