title: One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy
metadata.name: dc.contributor.author
metadata.value: Smirnov, A.N.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Aslanov, S.F.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Danilov, D.V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kurapova, O.Y.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Solovyeva, E.V.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2023-04-17T13:06:41Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2023-04-17T13:06:41Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2023-04-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: A.N. Smirnov, S.F. Aslanov, D.V. Danilov, O.Y. Kurapova, E.V. Solovyeva, One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy, Nanomaterials 13 (2023) 1267. https://doi.org/10.3390/nano13071267.
metadata.lang: en_GB
metadata.name: dc.identifier.issn
metadata.value: 2079-4991
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/41302
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Anisotropic gold nanoparticles have been recognized as promising agents for medical diagnostics and cancer therapy due to their wide functionality, photothermal effect and ability for optical signal amplification in the near-infrared range. In this work, a simple and rapid method for the preparation of bone-shaped gold nanoparticles coated with a dye-impregnated silica shell with an aminated surface is proposed. The possibility of further functionalization the nanostructures with a delivery vector using folic acid as an example is demonstrated. The average size of the resulted tags does not exceed 70 nm meeting the criteria of cell endocytosis. The prepared tags ehxibit surface-enhanced Raman scattering (SERS) spectra at excitation with lasers of 632.8 and 785 nm. Cell imaging is performed on HeLa cells based on the most pronounced SERS bands as tracking signal. The obtained images along with scanning electron microscopy of cell samples revealed the tendency of tags to agglomerate during endocytosis followed by the "hot spots" effect. To evaluate the toxic and proliferative effect of the nanotags, MTT assay was performed with two HeLa and HEP G2 cell lines. The results revealed higher viability for HEP G2 cells.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: Saint-Petersburg State University for support within the project 94031307 (part 3.2); Russian Science Foundation, grant № 22-73-10052 (parts 3.1, 3.3, 3.4)
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: MDPI
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: 13;1267
metadata.lang: -
metadata.name: dc.subject
metadata.value: gold nanoparticles
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: silicon oxide
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: one-pot synthesis
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: tag
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: SERS
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: bioimaging
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: cyanine
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: One-Pot Synthesis of Silica-Coated Gold Nanostructures Loaded with Cyanine 5.5 for Cell Imaging by SERS Spectroscopy
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 2023_Nanomaterials.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/41302/1/2023_Nanomaterials.pdf
resources.format: Adobe PDF
resources.description: Paper
resources.size: 2,94 MB