title: Two Novel Amyloid Proteins, RopA and RopB, from the Root Nodule Bacterium Rhizobium leguminosarum
metadata.name: dc.contributor.author
metadata.value: Kosolapova, A. O.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Belousov, M. V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sulatskaya, A. I.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Belousova, M. E.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sulatsky, M. I.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Antonets, K. S.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Volkov, K. V.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Lykholay, A. N.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shtark, O. Y.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Vasileva, E. N.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhukov, V. A.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Ivanova, A. N.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zykin, P. A.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kuznetsova, I. M.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Turoverov, K. K.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tikhonovich, I. A.
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Nizhnikov, A. A.
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2019-11-12T07:27:15Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2019-11-12T07:27:15Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-11-04
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 2218-273X
metadata.lang: -
metadata.name: dc.identifier.other
metadata.value: doi:10.3390/biom9110694
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/16561
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Amyloids represent protein fibrils with a highly ordered spatial structure, which not only cause dozens of incurable human and animal diseases but also play vital biological roles in Archaea, Bacteria, and Eukarya. Despite the fact that association of bacterial amyloids with microbial pathogenesis and infectious diseases is well known, there is a lack of information concerning the amyloids of symbiotic bacteria. In this study, using the previously developed proteomic method for screening and identification of amyloids (PSIA), we identified amyloidogenic proteins in the proteome of the root nodule bacterium Rhizobium leguminosarum. Among 54 proteins identified, we selected two proteins, RopA and RopB, which are predicted to have β-barrel structure and are likely to be involved in the control of plant-microbial symbiosis. We demonstrated that the full-length RopA and RopB form bona fide amyloid fibrils in vitro. In particular, these fibrils are β-sheet-rich, bind Thioflavin T (ThT), exhibit green birefringence upon staining with Congo Red (CR), and resist treatment with ionic detergents and proteases. The heterologously expressed RopA and RopB intracellularly aggregate in yeast and assemble into amyloid fibrils at the surface of Escherichia coli. The capsules of the R. leguminosarum cells bind CR, exhibit green birefringence, and contain fibrils of RopA and RopB in vivo.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by the Russian Science Foundation, grant 17-16-01100.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Biomolecules (MDPI)
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: 9;694
metadata.lang: -
metadata.name: dc.subject
metadata.value: amyloid
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: fibril
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: RopA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: RopB
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: outer membrane protein
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: porin
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: root nodule
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: bacteria
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: plant–microbial interaction
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Rhizobium leguminosarum
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Two Novel Amyloid Proteins, RopA and RopB, from the Root Nodule Bacterium Rhizobium leguminosarum
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 2019_biomolecules-09-00694.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/16561/1/2019_biomolecules-09-00694.pdf
resources.format: Adobe PDF
resources.description: Full text of article
resources.size: 18,96 MB