title: 3D-clinorotation induces specific alterations in metabolite profiles of germinating Brassica napus L. seeds
metadata.name: dc.contributor.author
metadata.value: Chantseva, Veronika
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bilova, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Smolikova, Galina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Frolov, Andrej
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Medvedev, Sergei
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2019-06-26T20:30:40Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2019-06-26T20:30:40Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Chantseva, V., Bilova, T., Smolikova, G., Frolov, A., and Medvedev, S. 2019. 3D-clinorotation induces specific alterations in metabolite profiles of germinating Brassica napus L. seeds. Bio. Comm. 64(1): 55–74.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi. org/10.21638/spbu03.2019.107
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/15793
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: During the whole history of their life on Earth, higher plants evolved under the constant gravity stimulus. Therefore, plants developed efficient mechanisms of gravity perception, underlying their ability to adjust the direction of growth to the gravity vector, i.e. the phenomenon of gravitropism. In this context, alterations in the magnitude and vector of the gravity field might compromise plant growth and development. This aspect was successfully addressed in gravity fields of low intensity (microgravity). On the other hand, microgravity can be simulated on the Earth by clinorotation, i.e. rotation of the experimental plant along one or several axes. This approach is routinely used for studies of gravity-related responses of crop plants, although the effect of simulated microgravity on the most sensitive ontogenetic stages - germination and seedling development - is still not sufficiently characterized. Recently, we addressed the effects of clinorotation on the proteome of germinating oilseed rape (Brassica napus) seeds. Here we extend this study to the seedling primary metabolome and address its changes in the presence of 3D-clinorotation. GC-MS analysis revealed essential alterations in patterns of sugars and sugar phosphates (specifically glucose-6-phosphate), methionine and glycerol. Thereby, abundances of individual metabolites showed high dispersion, indicating high lability and plasticity of the seedling metabolome.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by the Russian Foundation for Basic Research (Project No. 17-04-00862) and Saint Petersburg State University (Grant No. 7398/1, appendix No. 3, decision from 24.07.2018).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 64; Issue 1
metadata.lang: -
metadata.name: dc.subject
metadata.value: Brassica napus
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: clinorotation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: 3D-clinostat
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: metabolomics
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: primary metabolites
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: seed germination
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: simulated microgravity
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: 3D-clinorotation induces specific alterations in metabolite profiles of germinating Brassica napus L. seeds
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 3717-Article Text-10694-1-10-20190524.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/15793/1/3717-Article%20Text-10694-1-10-20190524.pdf
resources.format: Adobe PDF
resources.size: 1,12 MB