title: Genotyping pathogenic strains of genus Xanthomonas causing bacterioses in a number of plants by DDSL technique
metadata.name: dc.contributor.author
metadata.value: Lazarev, Alexander
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Terletskiy, Valeriy
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Chebotar, Vladimir
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2019-11-22T20:30:35Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2019-11-22T20:30:35Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2019-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Lazarev, A., Terletskiy, V., and Chebotar, V. 2019. Genotyping pathogenic strains of genus Xanthomonas causing bacterioses in a number of plants by DDSL technique. Bio. Comm. 64(3): 183–188.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2019.302
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/16623
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: In the genus Xanthomonas, specialists consider a significant number of species
  and varieties (pathovars) of phytopathogenic bacteria that infect many agricultural
  and ornamental plants (about 400 species), which leads to serious economic
  losses. For the timely detection of these pathogens, accurate diagnosis is
  necessary, allowing correct and prompt identification. Molecular genetic methods
  are able to identify populations of Xanthomonas strains with a fairly complete
  characterization of their hereditary material. The proposed method of
  genotyping — double digest and selective label (DDSL) — is based on the use of
  two restriction endonucleases for the separation of bacterial genomic DNA. The
  DNA polymerase (Taq) present in the reaction mixture along with biotinylated
  deoxycytosine triphosphate (Bio–dCTP) allows for the visualization of DNA fragments.
  The tag only labels DNA fragments that have 3’-recessed ends formed
  by the first enzyme (BcuI). The second restriction endonuclease (Eco147I) produces
  blunt ends that are unable to incorporate the label. As a result, in the
  DDSL reaction, 20–50 clearly distinguishable DNA fragments are visualized on
  the filter. The number and distribution of fragments are characteristic for each
  bacterial strain of the genus Xanthomonas. Genotyping these microorganisms
  makes it possible to identify the specific profile of each strain, i.e., assign it a
  sort of “bar code” for individual specification. The strains of bacteria of the genus
  Xanthomonas, obtained from different species (tomato, radish, sorghum)
  are genetically separated from each other, showing a specific pattern in terms
  of the distribution of DNA fragments, despite the common geographical origin.
  A comparatively rare case of the identity of strains, despite their geographical
  and temporal unrelatedness and different cultures, has been recorded.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was supported in
  sections by: 1. The state task (Project
  N 0665-2018-0006). Development of
  the ecological and genetic basis for the
  selection of antagonist microbial strains,
  entomopathogenic fungi and nematodes;
  development of technologies for the
  production and use of new multifunctional
  drugs to control the number of harmful
  organisms (pests, pathogens) and increase
  soil suppressiveness. 2. The comprehensive
  target program of the Russian Federation
  “Scientific support for the creation of a
  domestic sowing fund, products of plant
  protection for the production of competitive
  agricultural products by Russian producers,
  as well as the creation of production
  technologies (cultivation) and storage of
  such products for 2016–2025” (according to
  the priority direction “Potato”).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 64; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: Xanthomonas
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: strain
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: genotyping
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: double digest and selective label (DDSL)
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: restriction endonucleases
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Genotyping pathogenic strains of genus Xanthomonas causing bacterioses in a number of plants by DDSL technique
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 183-188.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/16623/1/183-188.pdf
resources.format: Adobe PDF
resources.size: 1,36 MB