title: Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms
metadata.name: dc.contributor.author
metadata.value: Zhukov, Vladimir
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zorin, Evgeny
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zhernakov, Aleksandr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Afonin, Alexey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Akhtemova, Gulnar
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bovin, Andrej
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Dolgikh, Aleksandra
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gorshkov, Artemii
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Gribchenko, Emma
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Ivanova, Kira
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kirienko, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kitaeva, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kliukova, Marina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kulaeva, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kusakin, Pyotr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Leppyanen, Irina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Pavlova, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Romanyuk, Daria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Rudaya, Elizaveta
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Serova, Tatiana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shtark, Oksana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sulima, Anton
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tsyganova, Anna
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Vasileva, Ekaterina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Dolgikh, Elena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tsyganov, Viktor
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tikhonovich, Igor
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-12-10T12:05:11Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-12-10T12:05:11Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Zhukov, V., Zorin, E., Zhernakov, A., Afonin, A., Akhtemova, G., Bovin, A., Dolgikh, A., Gorshkov, A., Gribchenko, E., Ivanova, K., Kirienko, A., Kitaeva, A., Kliukova, M., Kulaeva, O., Kusakin, P., Leppyanen, I., Pavlova, O., Romanyuk, D., Rudaya, E., Serova, T., Shtark, O., Sulima, A., Tsyganova, A., Vasileva, E., Dolgikh, E., Tsyganov, V., and Tikhonovich, I. 2021. Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms. Bio. Comm. 66(3): 181–197.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.301
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/33811
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The garden pea (Pisum sativum L.), like most members of Fabaceae family, is capable
  of forming symbioses with beneficial soil microorganisms such as nodule
  bacteria (rhizobia), arbuscular mycorrhizal (AM) fungi and plant growth promoting
  bacteria (PGPB). The autoregulation of nodulation (AON) system is known to
  play an important role in controlling both the number of nodules and the level
  of root colonization by AM via root-to-shoot signaling mediated by CLAVATA/
  ESR-related (CLE) peptides and their receptors. In the pea, mutations in genes
  Sym28 (CLV2-like) and Sym29 (CLV1-like), which encode receptors for CLE peptides,
  lead to the supernodulation phenotype, i.e., excessive nodule formation. The aim
  of the present study was to analyze the response of pea cv. ‘Frisson’ (wild type)
  and mutants P64 (sym28) and P88 (sym29) to complex inoculation with rhizobia,
  AM fungi and PGPB, with regard to biomass accumulation, yield and transcriptomic
  alterations. The plants were grown in quartz sand for 2 and 4 weeks after
  inoculation with either rhizobia (Rh) or complex inoculation with Rh + AM, Rh +
  PGPB, and Rh+AM+PGPB, and the biomass and yield were assessed. Transcriptome
  sequencing of whole shoots and roots was performed using a modified
  RNAseq protocol named MACE (Massive Analysis of cDNA Ends). In the experimental
  conditions, P88 (sym29) plants demonstrated the best biomass accumulation
  and yield, as compared to the wild type and P64 (sym28) plants, whereas
  P64 (sym28) had the lowest rate of biomass and seed yield. The transcriptome
  analysis showed that both supernodulating mutants more actively responded to
  biotic and abiotic factors than the wild-type plants and demonstrated increased
  expression of genes characteristic to late stages of nodule development. The
  roots of P64 (sym28) plants responded to AM+Rh treatment with upregulation of
  genes encoding plastid proteins, which can be connected with the activation of
  carotenoid biosynthesis (namely, the non-mevalonate pathway that takes place
  in root plastids). The more active response to symbionts in P88 (sym29) plants,
  as compared to cv. ‘Frisson’, was associated with counterregulation of transcripts
  involved in chloroplast functioning and development in leaves, which accompanies
  successful plant development in symbiotic conditions. Finally, the effect of
  retardation of plant aging upon mycorrhization on a transcriptomic level was recorded
  for cv. ‘Frisson’ but not for P64 (sym28) and P88 (sym29) mutants, which
  points towards its possible connection with the AON system. The results of this work link the plant’s autoregulation with the responsiveness
  to inoculation with beneficial soil microorganisms.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This study was supported by the Russian
  Science Foundation grant No 17-76-30016.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: RNAseq
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: transcriptomics
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: arbuscular mycorrhiza
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: nodule bacteria
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: complex inoculation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: autoregulation of nodulation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: garden pea
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea (Pisum sativum L.) under complex inoculation with beneficial microorganisms
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 9796-Article Text-38474-1-10-20211112.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/33811/1/9796-Article%20Text-38474-1-10-20211112.pdf
resources.format: Adobe PDF
resources.size: 16,43 MB