title: Genome Sequencing and Comparative Analysis of Saccharomyces cerevisiae Strains of the Peterhof Genetic Collection
metadata.name: dc.contributor.author
metadata.value: Drozdova, Polina B
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tarasov, Oleg V
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Matveenko, Andrew G
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Radchenko, Elina A
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Sopova, Julia V
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Polev, Dmitrii E
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Inge-Vechtomov, Sergey G
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Dobrynin, Pavel V
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2016-06-16T18:42:31Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2016-06-16T18:42:31Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2016-05-06
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.1371/journal.pone.0154722
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: PLoS One. 2016 May 6;11(5):e0154722.
metadata.lang: en_GB
metadata.name: dc.identifier.issn
metadata.value: 1932-6203
metadata.lang: -
metadata.name: dc.identifier.other
metadata.value: 10.1371/journal.pone.0154722
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/2304
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The Peterhof genetic collection of Saccharomyces cerevisiae strains (PGC) is a large laboratory stock that has accumulated several thousands of strains for over than half a century. It originated independently of other common laboratory stocks from a distillery lineage (race XII). Several PGC strains have been extensively used in certain fields of yeast research but their genomes have not been thoroughly explored yet. Here we employed whole genome sequencing to characterize five selected PGC strains including one of the closest to the progenitor, 15V-P4, and several strains that have been used to study translation termination and prions in yeast (25-25-2V-P3982, 1B-D1606, 74-D694, and 6P-33G-D373). The genetic distance between the PGC progenitor and S288C is comparable to that between two geographically isolated populations. The PGC seems to be closer to two bakery strains than to S288C-related laboratory stocks or European wine strains. In genomes of the PGC strains, we found several loci which are absent from the S288C genome; 15V-P4 harbors a rare combination of the gene cluster characteristic for wine strains and the RTM1 cluster. We closely examined known and previously uncharacterized gene variants of particular strains and were able to establish the molecular basis for known phenotypes including phenylalanine auxotrophy, clumping behavior and galactose utilization. Finally, we made sequencing data and results of the analysis available for the yeast community. Our data widen the knowledge about genetic variation between Saccharomyces cerevisiae strains and can form the basis for planning future work in PGC-related strains and with PGC-derived alleles.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: PBD acknowledges the Russian Foundation for Basic Research (www.rfbr.ru) for grant 14-04-31265. OVT and SGIV acknowledge the Russian Foundation for Basic Research for grant 15-29-02526. JVS acknowledges the Russian Science Foundation (www.rscf.ru) for grant 14-50-00069 and the Saint-Petersburg State University for grant 1.38.426.2015. PBD, AGM, EAR, and JVS acknowledge the Saint-Petersburg State University for research grant 1.37.291.2015. PBD and OVT acknowledge the Saint-Petersburg City Committee on Science and High School (knvsh.gov.spb.ru/) for grants 15404 and 15919, respectively. PBD, AGM, JVS, and SGIV acknowledge the Saint-Petersburg State University for research grant 15.61.2218.2013. PBD acknowledges the Saint-Petersburg State University for research grant 1.42.1394.2015.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: Public Library of Science
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: yeast
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Peterhof Genetic Collection
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: genome
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: whole genome sequencing
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Saccharomyces cerevisiae
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Genome Sequencing and Comparative Analysis of Saccharomyces cerevisiae Strains of the Peterhof Genetic Collection
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: journal.pone.0154722.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/2304/1/journal.pone.0154722.pdf
resources.format: Adobe PDF
resources.size: 3,26 MB