title: Natural antimicrobial peptide complexes in the fighting of antibiotic resistant biofilms: Calliphora vicina medicinal maggots
metadata.name: dc.contributor.author
metadata.value: Gordya, N
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Yakovlev, A
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kruglikova, A
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Tulin, D
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Potolitsina, E
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Suborova, T
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Bordo, D
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Rosano, C
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Chernysh, S
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2017-05-10T13:43:58Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2017-05-10T13:43:58Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2017-03-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Gordya N, Yakovlev A, Kruglikova A, Tulin D, Potolitsina E, Suborova T, et al. (2017) Natural antimicrobial peptide complexes in the fighting of antibiotic resistant biofilms: Calliphora vicina medicinal maggots. PLoS ONE 12(3): e0173559. doi:10.1371/journal.pone.0173559
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: eISSN 1932-6203
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/6251
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast
  majority of human bacterial infections and many severe complications such as chronic
  inflammatory diseases and cancer. Biofilms' resistance to the host immunity and antibiotics
  makes this kind of infection particularly intractable. Antimicrobial peptides (AMPs) are a ubiquitous
  facet of innate immunity in animals. However, AMPs activity was studied mainly on
  planktonic bacteria and little is known about their effects on biofilms. We studied structure
  and anti-biofilm activity of AMP complex produced by the maggots of blowfly Calliphora
  vicina living in environments extremely contaminated by biofilm-forming germs. The complex
  exhibits strong cell killing and matrix destroying activity against human pathogenic antibiotic
  resistant Escherichia coli, Staphylococcus aureus and Acinetobacter baumannii biofilms as
  well as non-toxicity to human immune cells. The complex was found to contain AMPs from
  defensin, cecropin, diptericin and proline-rich peptide families simultaneously expressed in
  response to bacterial infection and encoded by hundreds mRNA isoforms. All the families
  combine cell killing and matrix destruction mechanisms, but the ratio of these effects and
  antibacterial activity spectrum are specific to each family. These molecules dramatically
  extend the list of known anti-biofilm AMPs. However, pharmacological development of the
  complex as a whole can provide significant advantages compared with a conventional onecomponent
  approach. In particular, a similar level of activity against biofilm and planktonic
  bacteria (MBEC/MIC ratio) provides the complex advantage over conventional antibiotics.
  Available methods of the complex in situ and in vitro biosynthesis make this idea practicable.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: Russian Science Foundation, grant № 16-14-00048
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: PLOS One
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Biofilms, innate immunity, antimicrobial peptides, defensins, diptericins, cecropins, proline-rich peptides, naturally occurring antimicrobial peptide complex, blowflies, Calliphora vicina
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Natural antimicrobial peptide complexes in the fighting of antibiotic resistant biofilms: Calliphora vicina medicinal maggots
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Published version.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/6251/1/Published%20version.pdf
resources.format: Adobe PDF
resources.size: 2,43 MB