title: Wild-type p53 induced phosphatase sensitizes acute myeloid leukemia cells to conventional chemotherapy
metadata.name: dc.contributor.author
metadata.value: Golotin, Vasily
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Belotserkovskaya, Ekaterina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Girshova, Larisa
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Petukhov, Alexey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zaritsky, Andrey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Demidov, Oleg
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-12-10T18:07:53Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-12-10T18:07:53Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-09
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Golotin, V., Belotserkovskaya, E., Girshova, L., Petukhov, A., Zaritsky, A., and Demidov, O. 2021. Wild-type p53 induced phosphatase sensitizes acute myeloid leukemia cells to conventional chemotherapy. Bio. Comm. 66(3): 268–273.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.308
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/33822
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Recently wild-type p53-induced phosphatase was implicated in the pathogenesis
  of acute myeloid leukemia (AML) and “pre-leukemia” myeloproliferative
  conditions. Here we decided to check how the strategy directed to phosphatase
  inhibition affected sensitivity to conventional chemotherapy. All experiments
  were conducted on AML cell lines cultivated in vitro. The levels of wild-type
  p53-induced phosphatase vary in different AML cell lines. The chemical compound
  GSK2830371 reduced levels of phosphatase and diminished its activity.
  GSK2830371 did not significantly change the cell cycle distribution of AML
  cells when used alone or in combination with the anti-cancer chemotherapeutic
  drug Cytosar but increased caspase-dependent PARP1 cleavage. In contrast
  with previous studies, we did not observe the negative effect of phosphatase
  activity inhibition and depletion on cells when a chemical inhibitor was used as
  monotherapy. Using a combination of GSK2830371 with Cytosar we were able
  to reduce the threshold of chemotherapy-dependent cytotoxicity and more efficiently
  eliminate leukemic cells. We propose considering inhibition of wildtype
  p53-induced phosphatase as a prospective strategy in improving anti-AML
  therapy.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The research was supported by a
  grant from the Russian Science Foundation
  19-75-20128.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 3
metadata.lang: -
metadata.name: dc.subject
metadata.value: leukemia
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: AML
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: chemotherapy
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: cytarabine
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Wild-type p53 induced phosphatase sensitizes acute myeloid leukemia cells to conventional chemotherapy
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 9873-Article Text-38473-1-10-20211112.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/33822/1/9873-Article%20Text-38473-1-10-20211112.pdf
resources.format: Adobe PDF
resources.size: 1,76 MB