title: Personalized medicine: the role of sequencing technologies in diagnostics, prediction and selection of treatment of monogenous and multifactorial diseases
metadata.name: dc.contributor.author
metadata.value: Glotov, Oleg
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Chernov, Alexandr
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Fedyakov, Michael
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Larionova, Valentina
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Zaretsky, Andrey
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Donnikov, Maksim
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Glotov, Andrey
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2023-01-27T12:18:05Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2023-01-27T12:18:05Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2022-12
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Glotov, O., Chernov, A., Fedyakov, M., Larionova, V., Zaretsky, A., Donnikov, M., and Glotov, A. 2022. Personalized medicine: the role of sequencing technologies in diagnostics, prediction and selection of treatment of monogenous and multifactorial diseases. Bio. Comm. 67(4): 266–285. https://doi.org/10.21638/spbu03.2022.403
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2022.403
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/38900
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: The review highlights various methods for deciphering the nucleotide sequence
  (sequencing) of nucleic acids and their importance for the implementation of
  the three main principles of personalized medicine: prevention, predictability
  and personalization. The review, along with its own practical examples, considers
  three generations of sequencing technologies: 1) sequencing of cloned or
  amplified DNA fragments according to Sanger and its analogues; 2) massive
  parallel sequencing of DNA libraries with short reads (NGS); and 3) sequencing
  of single molecules of DNA and RNA with long reads. The methods of whole genome,
  whole exome, targeted, RNA sequencing and sequencing based on chromatin
  immunoprecipitation are also discussed. The advantages and limitations
  of the above methods for diagnosing monogenic and oncological diseases, as
  well as for identifying risk factors and predicting the course of socially significant
  multifactorial diseases are discussed. Using examples from clinical practice,
  algorithms for the application and selection of sequencing technologies
  are demonstrated. As a result of the use of sequencing technologies, it has now
  become possible to determine the molecular mechanism of the development
  of monogenic, orphan and multifactorial diseases, the knowledge of which
  is necessary for personalized patient therapy. In science, these technologies
  paved the way for international genome projects — the Human Genome Project,
  the HapMap, 1000 Genomes Project, the Personalized Genome Project, etc.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: Supported by the Ministry
  of Science and Higher Education of
  Russian Federation (project “Multicenter
  research bioresource collection “Human
  Reproductive Health” contract No. 075-
  15-2021-1058 from September 28, 2021).
  The reported study was funded by
  Foundation for Scientific and Technological
  Development of Yugra according to the
  research project No. 2022-05-04.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 67; Issue 4
metadata.lang: -
metadata.name: dc.subject
metadata.value: sequencing technologies
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: whole genome sequencing
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: whole exome sequencing
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: advantages
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: limitations
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: personalized medicine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: human genomic projects
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: diagnostics
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: disease prediction
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: personalized therapy
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Personalized medicine: the role of sequencing technologies in diagnostics, prediction and selection of treatment of monogenous and multifactorial diseases
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 03.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/38900/1/03.pdf
resources.format: Adobe PDF
resources.size: 3,93 MB