title: Coordinated alterations in gene expression and metabolomic profiles of Chlamydomonas reinhardtii during batch autotrophic culturing
metadata.name: dc.contributor.author
metadata.value: Puzanskiy, Roman
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Romanyuk, Daria
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Shishova, Maria
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2018-07-09T13:47:36Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2018-07-09T13:47:36Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2018-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Puzanskiy, R., Romanyuk, D., and Shishova, M. 2018. Coordinated alterations in gene expression and metabolomic profiles of Chlamydomonas reinhardtii during batch autotrophic culturing. Bio. Comm. 63(1): 87–99.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: 10.21638/spbu03.2018.110
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/10380
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Chlamydomonas reinhardtii was grown under autotrophic batch culturing, which
  is known to be a widely applicable method for both fundamental research and
  applied purposes. This type of cultivation results in elevation of cell density and
  fast exhaustion of nutrient resources. We expected the revealed metabolic adaptation
  to be triggered at the transcriptional level. This investigation focuses on
  analyzing expression of the genes encoding enzymes involved in primary metabolism
  and plastid transporters during the exponential phase of C. reinhardtii autotrophic
  batch culture. About two-thirds of the tested genes demonstrated differential
  expression during algae growth. Patterns of expression were clustered into
  5 groups. Most of the genes were gathered in two large clusters, characterized
  by peaks of expression at early or later exponential growth (EG). Genes which
  showed maximal expression in early EG were OMT1, HXK1, AMYB1, ACK1,2, CHLREDRAFT_
  123419, APE2, PCK1, CHLREDRAFT_195672, CIS2, TPT2 and ACLA1. Among the
  genes with maximal expression in later EG were SBE3, TPIC, CHLREDRAFT_137300,
  CHLREDRAFT_111372, PPT1 and CHLREDRAFT_122970. There were no genes detected
  with maximal expression at the cessation of proliferation. PCA showed that
  the expression profiles in the beginning EG were similar, and profiles changed
  drastically in the middle of exponential growth. PLS-DA revealed the difference
  between the beginning of EG and later periods linked to PC1 (44%), between late
  EG and early stationary linked to PC2 (23%) and finally between two points at
  the beginning of growth linked to PC3 (10%). Mapping of genes and metabolites
  according to their correlation revealed a graph with two clusters. The first, smaller
  cluster contains genes that encode plastid exporters, enzymes of starch and
  carbohydrates metabolism. The expression level of these genes peaked later in
  EG. These genes are mainly associated with metabolites such as carbohydrates,
  acylglycerols and fatty acids metabolism. The second cluster is larger and more
  diverse. It combines genes with maximum expression in the beginning of EG. The
  core of this cluster is formed by genes encoding enzymes of fatty acids synthesis,
  energy and plastic pathways, and plastid transporters. This cluster included the
  majority of amino acids, carboxylic acids and many fatty acids.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: The work was partly supported by
  the Russian Foundation for Basic Research
  (No. 16-34-01122, 16-04-00743).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Communications;Volume 63; Issue 1
metadata.lang: -
metadata.name: dc.subject
metadata.value: gene expression
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: primary metabolism
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: autotrophic growth
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: plastid transporter
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: Chlamydomonas reinhardtii
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: batch culture
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: PCA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: PLS
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: MEBA
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: exponential growth
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Coordinated alterations in gene expression and metabolomic profiles of Chlamydomonas reinhardtii during batch autotrophic culturing
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 10-Puzanskiy.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/10380/1/10-Puzanskiy.pdf
resources.format: Adobe PDF
resources.size: 4,14 MB