title: Encapsulation of rat brain slices in agar gel solution for long-term and reversible cryopreservation
metadata.name: dc.contributor.author
metadata.value: Mokrushin, Anatoly
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2023-06-15T20:34:52Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2023-06-15T20:34:52Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2023-03
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Mokrushin, A. 2023. Encapsulation of rat brain slices in agar gel solution for long-term and reversible cryopreservation. Bio. Comm. 68(1): 21–29. https://doi.org/10.21638/spbu03.2023.103
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2023.103
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/41584
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Earlier there was found activity of glutamatergic ionotropic α-amino-3-hydroxy-
  5-methylisoxazole-4-propionic acid (AMPAR) and N-methyl-D-aspartate receptors
  (NMDAR) disturbed after prolonged cryopreservation of brain slices at
  temperature of –10 °C. To eliminate cryodamage of AMPAR and NMDAR, the
  slices were encapsulated in the special freezing solution (SFS). SFS consisted
  of agar at various concentrations (33, 44 and 50 %) and artificial cerebrospinal
  fluid (67, 56 and 50 %, respectively). This solution was used for long-term
  cryopreservation of slices (52 days, –10 °C). Alterations in amplitudes of AMPA
  and NMDA potentials in the slices after rewarming were studied. Recovery of
  AMPAR and NMDAR after cryopreservation of the slices in SFS was the most
  optimal when the agar concentration in SFS was 50 %. Cryopreservation of the
  slices in SFS with different agar concentrations predominantly promoted the
  development of long-term potentiation in 78 % of the tested slices. Thus, the
  encapsulation of brain slices in SFS contributes to the preservation of AMPAR
  and NMDAR activity during long-term and reversible cryopreservation.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 68; Issue 1
metadata.lang: -
metadata.name: dc.subject
metadata.value: brain slices
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: cryopreservation
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: freezing/rewarming
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: agar
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Encapsulation of rat brain slices in agar gel solution for long-term and reversible cryopreservation
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 13190-Article Text-53885-1-10-20230502.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/41584/1/13190-Article%20Text-53885-1-10-20230502.pdf
resources.format: Adobe PDF
resources.size: 1,15 MB