title: Supply of acetyl-CoA to N2-fixing bacteroids: insights from the mutational and proteomic analyses of Sinorhizobium meliloti
metadata.name: dc.contributor.author
metadata.value: Onishchuk, Olga
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kurchak, Oxana
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Chizhevskaya, Elena
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Simarov, Boris
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Provorov, Nikolay
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2021-08-20T19:49:07Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2021-08-20T19:49:07Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2021-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Onishchuk, O., Kurchak, O., Chizhevskaya, E., Simarov, B., and Provorov, N. 2021. Supply of acetyl-CoA to N2-fixing bacteroids: insights from the mutational and proteomic analyses of Sinorhizobium meliloti. Bio. Comm. 66(2): 124–128.
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2021.203
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/32814
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Rhizobia represent a diverse group of gram-negative bacteria capable of fixing
  atmospheric nitrogen in symbiosis with leguminous plants. Mechanisms of
  symbiotic efficiency are important to study not only to reveal the “fine tuning”
  of the host–symbiont supra-organismal genetic system emergence, but also to
  develop agriculture with minimal environmental risks. In this paper we demonstrate
  that among seven genes whose inactivation by Tn5 insertions results in
  an increased efficiency of rhizobia (Sinorhizobium meliloti) symbiosis with alfalfa
  (Eff++ phenotype), six genes are involved in the metabolism of small molecules.
  One of them (SMc04399) encodes for acetate-CoA transferase catalyzing the
  formation of acetyl-CoA from acyl-CoA. Since acetyl-CoA is required for operation
  of the Krebs cycle, providing ATP for symbiotic N2 fixation, we suggest
  that a significant portion of this coenzyme utilized by bacteroids is provided
  by the plant cell supporting the energy-consuming nitrogenase reaction. Proteomic
  data analysis allow us to reveal the lability of enzymatic pathways which
  are involved in bacteroids in the production and catabolism of acetyl-CoA and
  which should be modified to obtain the Eff++ phenotype. This phenotype was
  developed also after inactivation of NoeB protein which is involved in the hostspecific
  nodulation and is characterized by an elevated production in wild type
  S. meliloti bacteroids, suggesting a multifunctional role of noeB in the symbiosis
  operation.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This research was supported by
  Russian Science Foundation, Grant 19-16-
  00081.
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 66; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: Sinorhizobium meliloti
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: symbiotic efficiency
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: metabolism of small molecules
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: acetyl CoA, acetate-CoA transferase
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: transposon (Tn5) mutagenesis
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: proteomic analysis
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Supply of acetyl-CoA to N2-fixing bacteroids: insights from the mutational and proteomic analyses of Sinorhizobium meliloti
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: 124-128.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/32814/1/124-128.pdf
resources.format: Adobe PDF
resources.size: 1,12 MB