title: Acetamidase gene as a new plasmid marker for CRISPR modification of industrial and laboratory Saccharomyces yeasts
metadata.name: dc.contributor.author
metadata.value: Urakov, Valery
metadata.lang: -
metadata.name: dc.contributor.author
metadata.value: Kushnirov, Vitaly
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2024-10-11T17:23:49Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2024-10-11T17:23:49Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2024-06
metadata.lang: -
metadata.name: dc.identifier.citation
metadata.value: Urakov, V. and Kushnirov, V. 2024. Acetamidase gene as a new plasmid marker for CRISPR modification of industrial and laboratory Saccharomyces yeasts. Bio. Comm. 69(2): 105–110. https://doi.org/10.21638/spbu03.2024.205
metadata.lang: en_GB
metadata.name: dc.identifier.other
metadata.value: https://doi.org/10.21638/spbu03.2024.205
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/47686
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Genetic manipulation of laboratory yeast strains relies on the use of auxotrophic
  markers. However, such markers are usually missing and undesirable in
  industrial yeasts. The standard option is then to use antibiotic resistance markers
  such as KanMX. However, the required antibiotic concentration can vary
  significantly depending on the growth medium and yeast strain, often resulting
  in a high proportion of false-positive colonies. An alternative selection procedure
  could be based on the ability of yeast cells to utilize an uncommon source
  of nitrogen. Many yeasts, including Saccharomyces, are unable to utilize acetamide.
  This ability can be conferred by a single acetamidase gene. The CRISPR/
  Cas9 technology has an advantage over previous methods of yeast modification
  in that it allows genomic modifications to be introduced without leaving a
  selectable marker in the genome. Driven by these two motives, we constructed
  a CRISPR/Cas9 plasmid with an acetamidase gene from the fungus Aspergillus
  nidulans, which allows the selection of yeast transformants on medium with
  acetamide as sole nitrogen source, and tested this plasmid with a triploid wine
  strain and a haploid laboratory strain.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This work was supported by the
  Ministry of Science and Higher Education of
  the Russian Federation (agreement 075-15-
  2021-1071).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: St Petersburg State University
metadata.lang: en_GB
metadata.name: dc.relation.ispartofseries
metadata.value: Biological Сommunications;Volume 69; Issue 2
metadata.lang: -
metadata.name: dc.subject
metadata.value: wine yeast
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: selective marker
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: acetamidase gene
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: CAR1 gene
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: CRISPR/ Cas9
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: acetamide
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: arginine
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: nitrogen source
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Acetamidase gene as a new plasmid marker for CRISPR modification of industrial and laboratory Saccharomyces yeasts
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Bio-Comm_69_2_105-110.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/47686/1/Bio-Comm_69_2_105-110.pdf
resources.format: Adobe PDF
resources.size: 1,37 MB