title: Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
metadata.name: dc.contributor.author
metadata.value: Kostyuchenko, Roman P
metadata.lang: -
metadata.name: dc.date.accessioned
metadata.value: 2022-06-20T14:08:22Z
metadata.lang: -
metadata.name: dc.date.available
metadata.value: 2022-06-20T14:08:22Z
metadata.lang: -
metadata.name: dc.date.issued
metadata.value: 2022
metadata.lang: -
metadata.name: dc.identifier
metadata.value: 10.3390/genes13020270
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.3390/genes13020270
metadata.lang: -
metadata.name: dc.identifier.issn
metadata.value: 10.3390/genes13020270
metadata.lang: -
metadata.name: dc.identifier.uri
metadata.value: http://hdl.handle.net/11701/36864
metadata.lang: -
metadata.name: dc.description.abstract
metadata.value: Nanos is a translational regulator that is involved in germline development in a number of diverse animals and is also involved in somatic patterning in several model organisms, including insects. Neither germline development nor somatic stem cell lines/undifferentiated multipotent cells have been characterized in the development of the annelid Alitta virens, nor is the mechanism of germ/stem-line specification generally well-understood in annelids. Here, I have cloned an Avi-nanos ortholog from A. virens and determined the spatial and temporal expression of Nanos. The results revealed that transcripts of nanos are expressed during differentiation of multiple tissues, including those that are derived from the 2d and 4d cells. In late embryonic stages and during larval development, these transcripts are expressed in the presumptive brain, ventral nerve cord, mesodermal bands, putative primordial germ cells (PGCs), and developing foregut and hindgut. During metamorphosis of the nectochaete larva into a juvenile worm, a posterior growth zone consisting of nanos-positive cells is established, and the PGCs begin to migrate. Later, the PGCs stop migrating and form a cluster of four nanos-expressing cells located immediately behind the jaws (segments 4-5). During posterior regeneration following caudal amputation, a robust Avi-nanos expression appears de novo at the site of injury and further accompanies all steps of regeneration. The obtained data suggest that blastemal cells are mostly derived from cells of the segment adjacent to the amputation site; this is consistent with the idea that the cluster of PGCs do not participate in regeneration.
metadata.lang: en_GB
metadata.name: dc.description.sponsorship
metadata.value: This research was funded by RSF grant 17-14-01089 (gene identification, bioinformatics analysis) and RFBR grant 19-04-01111-a (gene cloning and expression analysis).
metadata.lang: en_GB
metadata.name: dc.language.iso
metadata.value: en
metadata.lang: en_GB
metadata.name: dc.publisher
metadata.value: MDPI
metadata.lang: en_GB
metadata.name: dc.subject
metadata.value: annelids; Nereis; mesoderm; primordial germ cells; PGCs migration; somatic stem cells; neural system; posterior growth zone; foregut; hindgut; regeneration; evolution
metadata.lang: en_GB
metadata.name: dc.title
metadata.value: Nanos Is Expressed in Somatic and Germline Tissue during Larval and Post-Larval Development of the Annelid Alitta virens
metadata.lang: en_GB
metadata.name: dc.type
metadata.value: Article
metadata.lang: en_GB
resources.filename: Kostyuchenko_Genes_2022.pdf
resources.url: https://dspace.spbu.ru/bitstream/11701/36864/1/Kostyuchenko_Genes_2022.pdf
resources.format: Adobe PDF
resources.size: 4,08 MB